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IB47010 Gel/PCR DNA Fragment Extraction Sample Kit for 4 Preparations
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GBP 41
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What Stands Out
Product Details
- Kit includes (4) DF columns, (4) 2mL collection tubes, 3mL DF buffer, 1mL wash buffer (4mL add ethanol) and 1mL elution buffer
- For DNA fragments (100 bp-10 Kb) from agarose gel, PCR, or other enzymatic reactions
- Spin column format with 10 microgram DNA binding capacity
- Expectant yield is 80-90 percent for gel extraction and 90-95 percent for PCR clean-up
- Applications include PCR fluorescent or radioactive sequencing, restriction digestion, DNA labeling, ligation
- Operation time: 15 min for PCR clean-up, 20 min for gel extraction, with 20-50 microliter elution volume
Who Should Buy?
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Molecular Biologists
Ideal for molecular biologists who require efficient DNA purification for analysis and cloning in research settings.
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Students
Suitable for students conducting experiments in genetics or microbiology labs needing reliable DNA extraction kits.
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Small Laboratories
Perfect for small labs needing cost-effective solutions for DNA fragment extraction without compromising quality.
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High-Volume Users
Not suitable for facilities requiring high-throughput DNA extraction due to limited sample preparation capacity.
Product Description
IB47010 Gel/PCR DNA Fragment Extraction Sample Kit for 4 Preparations
About This Item
Introducing the IBI Scientific IB47010 GelPCR DNA Fragment Extraction Sample Kit for 4 Preparations. This kit is a must-have tool for anyone working with agarose gel, PCR, or other enzymatic reactions. With the Gel/PCR DNA Fragments Extraction Kit, you can easily recover or concentrate DNA fragments ranging from 100 bp to 10 Kb.
The unique design of this kit ensures efficient extraction and purification of the DNA fragments without the need for phenol extraction or alcohol precipitation. Using chaotropic salt, the Gel/PCR DNA Fragments Extraction Kit dissolves agarose gel and denatures enzymes, allowing the DNA fragments to be bound by the glass fiber matrix of the spin column. Contaminants are easily removed with the Wash Buffer, which contains ethanol, while the purified DNA fragments are eluted using a low salt Elution Buffer or TE. This comprehensive kit is not only time-efficient, with the entire procedure taking only 20 minutes, but it also provides excellent recovery rates - up to 90 percent for Gel Extraction and up to 95 percent for PCR Cleanup. Once purified, the DNA fragments are ready for immediate use in a variety of applications, including PCR, fluorescent or radioactive sequencing, restriction enzyme digestion, DNA labeling, and ligation. What sets the Gel/PCR DNA Fragments Extraction Kit apart is its ability to deliver high recoveries for both small base pair DNA fragments (50-200 bp) and large base pair DNA fragments (greater than 8 Kb).
Each batch of the Gel/PCR DNA Fragments Extraction Kit is tested extensively for quality assurance, ensuring reliable performance and consistent results. Please note that the DF buffer in this kit contains guanidine thiocyanate, which is a harmful irritant. It is important to always wear a lab coat, disposable gloves, and protective goggles when using this kit to ensure your safety. If you are looking for a reliable and efficient solution for your DNA fragment extraction needs, look no further than the IBI Scientific IB47010 GelPCR DNA Fragment Extraction Sample Kit for 4 Preparations. Order yours today and experience the convenience and effectiveness of this high-quality product.
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GBP 41
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