HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic Acid)], 100 Grams
GBP 83
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A versatile solution with a wide pH range and low heavy metal content for optimal performance in various applications.
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| Item Weight | 0.22 lbs (100 grams) |
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Who Should Buy?
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Biological Researchers
Ideal for researchers conducting in vitro experiments requiring stable pH conditions for cell cultures and biochemical assays.
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Pharmaceutical Development
Beneficial for pharmaceutical companies focusing on drug formulation that demands precise pH buffering agents during testing.
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University Laboratories
Suitable for academic labs needing reliable pH buffers for educational experiments and student research projects.
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Casual Users
Not suitable for individuals without a scientific background who do not require precise buffer solutions.
Product Description
HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic Acid)], 100 Grams
About This Item
Introducing HEPES, Free Acid [N-2-Hydroxyethyl piperazine N-2-ethanesulfonic Acid], 100 Grams Are you in need of a reliable buffer for your laboratory experiments and research? Look no further! Ubuy presents HEPES, a zwitterionic N-substituted aminosulfonic acid buffer that is essential for maintaining stable pH levels in various biological applications. HEPES, also known as N-2-Hydroxyethyl piperazine N-2-ethanesulfonic Acid, is a versatile compound widely used in laboratory settings. It is a highly effective buffering agent with a pKa of 7.5 at 25°C, making it ideal for maintaining optimal pH in a variety of experimental conditions. With a pH range of 6.8 - 8.2, this HEPES buffer ensures accurate and consistent results. It acts as a powerful regulator, stabilizing the pH level and maintaining it within the desired range.
This not only enhances the accuracy of your experiments but also promotes the viability and functionality of your biological samples. In addition to its pH control properties, HEPES is also known for its low ionic strength and minimal toxicity. It does not interfere with enzyme activities or alter the behavior of proteins, making it a trusted choice in various biochemistry and molecular biology applications. This pack includes 100 grams of HEPES, providing you with an ample supply for your laboratory needs. As a reputable ecommerce store, Ubuy ensures the quality and purity of our products, meeting the highest industry standards. Don't compromise on the success of your research.
Choose HEPES, the trusted choice of scientists and researchers worldwide. Purchase your 100 grams of HEPES, Free Acid [N-2-Hydroxyethyl piperazine N-2-ethanesulfonic Acid] from Ubuy today and take your experiments to the next level!.
Customer Questions & Answers
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Question:
What is HEPES, Free Acid used for?
Answer: HEPES, Free Acid is a widely used buffering agent in biological and biochemical research. Its fundamental role is to maintain a stable pH in solutions, making it essential for various applications like cell culture, enzyme assays, and electrophoresis. It ensures that experiments remain consistent and reproducible, particularly in sensitive applications such as protein studies and nucleotide work. -
Question:
Is HEPES, Free Acid safe to handle?
Answer: Yes, HEPES, Free Acid is generally considered safe to handle when proper laboratory safety precautions are followed. It is important to wear gloves and safety goggles while manipulating the powder, as inhalation or contact with eyes can lead to irritation. For most users, adhering to standard laboratory safety protocols will ensure a safe working environment while using this versatile compound. -
Question:
How should HEPES, Free Acid be stored?
Answer: To maintain its effectiveness, HEPES, Free Acid should be stored in a cool, dry place, away from direct sunlight and moisture. The container should be tightly sealed to prevent any contamination or degradation. Proper storage conditions help extend the shelf life and ensure that it remains stable for your laboratory applications. -
Question:
Can HEPES, Free Acid be used in cell culture?
Answer: Absolutely! HEPES, Free Acid is frequently utilized in cell culture media to maintain an optimal pH level, particularly in cells sensitive to pH changes. Its buffering capacity is especially useful in applications involving cultured cells that require a stable environment for growth and experimentation. It helps in enhancing cellular functions and viability during your experiments. -
Question:
What are the differences between HEPES and other buffering agents?
Answer: HEPES offers several advantages over traditional buffering agents like bicarbonate or phosphate. It provides a consistent pH range and is less susceptible to pH changes in varying environmental conditions. Furthermore, HEPES does not interfere with biological reactions and is bio-compatible, making it a preferred choice in various biochemical protocols. -
Question:
What is the optimum pH range for HEPES, Free Acid?
Answer: HEPES, Free Acid effectively buffers a pH range of approximately 6.8 to 8.2. This range is particularly beneficial for many biological systems and biochemical applications. By maintaining the pH within this range, users can enhance the reliability and reproducibility of their experiments, ensuring that results are accurate and relevant. -
Question:
Can HEPES, Free Acid be used in molecular biology experiments?
Answer: Yes, HEPES, Free Acid is extensively used in molecular biology experiments. Its buffering capacity is crucial for maintaining optimal conditions during reactions, such as PCR amplification and DNA/RNA isolation. By providing a stable environment, HEPES helps ensure the integrity of nucleic acids during amplification processes. -
Question:
What is the solubility of HEPES, Free Acid in water?
Answer: HEPES, Free Acid is highly soluble in water, with solubility increasing with temperature. Typically, it dissolves well at room temperature and is easily incorporated into various aqueous solutions used in scientific experiments. This high solubility makes it convenient for laboratory use and allows researchers to prepare solutions without significant effort. -
Question:
Where can I buy HEPES, Free Acid [N-2-Hydroxyethyl piperazine N'-2-ethanesulfonic Acid], 100 Grams?
Answer: You can buy HEPES, Free Acid [N-2-Hydroxyethyl piperazine N'-2-ethanesulfonic Acid], 100 Grams from Ubuy, which is a reliable online platform that offers a variety of laboratory supplies and reagents in UK. Ubuy provides easy access to high-quality chemicals and ensures you find what you need for your research or laboratory applications.
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