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Hyaluronidase Inhibitor Screening Assay Kit (DIHY-100)
GBP 410
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Price
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If Item price + shipping is below GBP 135, 20% VAT will be calculated on checkout, if it is equal or above GBP 135, no VAT will be charged. )
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Rapid and reliable. Entire assay can be completed in 30 min.
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What Stands Out
Product Details
- Rapid and reliable assay can be completed in 30 min
- Simple and convenient procedure with enzymatic reaction and stop reagent
- Robust and amenable to high-throughput screening (HTS)
- Can be easily automated on HTS liquid handling systems
- Measures hyaluronidase activity by hydrolyzing hyaluronic acid
- Decrease in turbidity at 600 nm is directly proportional to hyaluronidase activity
| Item Weight | 1.5 lbs (680 grams) |
Who Should Buy?
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Research Laboratories
Ideal for labs conducting research on hyaluronidase inhibitors for therapeutic purposes and pharmaceutical development.
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Biotech Companies
Beneficial for biotech firms focusing on drug discovery and development related to hyaluronidase activities.
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Academic Institutions
Useful for universities and colleges exploring enzymatic pathways and their inhibitors in biochemistry courses and research.
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General Public
Not suitable for non-professionals due to the technical complexity and requirement for specialized knowledge in biochemistry.
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Basic Academic Use
Inappropriate for basic coursework that does not involve advanced biochemistry or enzyme research methodologies.
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Non-Research Settings
Not intended for non-research environments such as routine clinical applications or general medical use without specific study.
Product Description
Hyaluronidase Inhibitor Screening Assay Kit (DIHY-100)
About This Item
Looking for a reliable and efficient solution to measure hyaluronidase activity? Look no further than the Hyaluronidase Inhibitor Screening Assay Kit DIHY-100 from BioAssay Systems. Hyaluronidases are a group of enzymes that play a crucial role in breaking down hyaluronic acid, a key component of the extracellular matrix in organisms. Understanding the activity of these enzymes is essential for research related to cell proliferation and migration. BioAssay Systems' Hyaluronidase Inhibitor Screening Assay Kit offers a two-step turbidimetric reaction method to accurately measure hyaluronidase activity. This kit assesses the amount of hyaluronic acid hydrolyzed by the enzyme, providing valuable insights into its activity level. To ensure precise results, a stop reagent is included in the kit, which halts the enzymatic reaction and creates turbidity with any remaining hyaluronic acid in the well.
By measuring the decrease in turbidity at 600 nm, the assay kit allows direct quantification of hyaluronidase activity in the sample. Precision and accuracy are the hallmarks of the Hyaluronidase Inhibitor Screening Assay Kit DIHY-100. By using this kit, you can effectively screen and evaluate potential hyaluronidase inhibitors in your research. Don't miss out on the opportunity to advance your research. Choose the Hyaluronidase Inhibitor Screening Assay Kit DIHY-100 from ubuy today and unlock new possibilities in your scientific investigations.
Customer Questions & Answers
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Question:
What is the purpose of the Hyaluronidase Inhibitor Screening Assay Kit (DIHY-100)?
Answer: The Hyaluronidase Inhibitor Screening Assay Kit (DIHY-100) is designed to identify and evaluate the effectiveness of potential hyaluronidase inhibitors. Hyaluronidase is an enzyme that breaks down hyaluronic acid, affecting tissue hydration and cellular processes. This assay kit allows researchers to screen compounds for their inhibiting abilities, which can be pivotal in therapeutic developments targeting conditions like cancer, arthritis, or skin disorders. -
Question:
Who can benefit from using the Hyaluronidase Inhibitor Screening Assay Kit?
Answer: Researchers and scientists in pharmacology, biochemistry, and clinical laboratories can greatly benefit from this kit. It is specifically useful for those studying the effects of hyaluronidase in various pathological conditions. Additionally, pharmaceutical companies involved in drug development can utilize this kit for screening candidates that may be explored as potential therapeutic agents against diseases characterized by excessive hyaluronidase activity. -
Question:
What are the key components included in the DIHY-100 kit?
Answer: The Hyaluronidase Inhibitor Screening Assay Kit (DIHY-100) typically includes purified hyaluronidase enzyme, substrate solutions, and reaction buffer, along with a detailed protocol for each experiment. These components are essential for conducting accurate and reproducible assays. Furthermore, having a reliable protocol ensures that users can efficiently perform the screening process, leading to quicker results and insights into hyaluronidase inhibition. -
Question:
How do you perform the assay using the Hyaluronidase Inhibitor Screening Assay Kit?
Answer: To perform the assay, you'll first prepare the reaction mixture by combining the provided hyaluronidase enzyme and substrate under specified conditions. Following this, incubate the mixture and measure the reaction's absorbance. This procedure will allow you to quantify inhibition levels of different compounds on hyaluronidase activity. By comparing results against controls, you can determine the effectiveness of various inhibitors, which is crucial for further pharmacological studies. -
Question:
Can the DIHY-100 kit be used for high-throughput screening?
Answer: Yes, the Hyaluronidase Inhibitor Screening Assay Kit (DIHY-100) is designed to accommodate high-throughput screening methods. Researchers often need to test multiple compounds simultaneously, and this kit provides the flexibility to conduct numerous assays in parallel. This approach saves time and resources while increasing the efficiency of identifying promising inhibitors, making it invaluable in drug discovery projects. -
Question:
What are the expected results when using the DIHY-100 kit?
Answer: Users can expect to obtain quantitative results regarding the inhibition of hyaluronidase activity when using the Hyaluronidase Inhibitor Screening Assay Kit (DIHY-100). The results will be reflected in absorbance readings that correlate to hyaluronidase activity levels. With this quantitative data, researchers can effectively determine how well different compounds inhibit the enzyme, paving the way for further experimental or clinical investigations. -
Question:
Is prior laboratory experience required to use this assay kit?
Answer: While prior laboratory experience is beneficial, the Hyaluronidase Inhibitor Screening Assay Kit (DIHY-100) comes with a comprehensive protocol that guides users through the process. Researchers new to enzymatic assays can follow the instructions closely to achieve reliable results. For best outcomes, it is recommended to have some familiarity with basic laboratory techniques, such as pipetting and absorbance measurements, as well as an understanding of enzyme kinetics. -
Question:
Are there any safety considerations when using the DIHY-100 kit?
Answer: Yes, safety should always be a priority when working with assay kits like the Hyaluronidase Inhibitor Screening Assay Kit (DIHY-100). Users should wear appropriate personal protective equipment (PPE), including gloves and safety goggles. It is also important to handle all reagents according to the safety data sheets provided. Being aware of potential hazards ensures a safe working environment while performing the assays. -
Question:
Where can I buy the Hyaluronidase Inhibitor Screening Assay Kit (DIHY-100) in UK?
Answer: You can purchase the Hyaluronidase Inhibitor Screening Assay Kit (DIHY-100) through Ubuy, a reliable e-commerce platform that offers various scientific and laboratory products tailored to your needs in UK. Ubuy ensures easy accessibility to the kit, providing a streamlined shopping experience with comprehensive product information and customer support.
BioAssay Systems Assay Kits Editorial Review
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GBP 410
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Features & Benefits
- Rapid and reliable assay
- Completed in 30 minutes
- Simple and convenient procedure
- No wash or reagent transfer steps involved
- Robust and amenable to HTS
- Can be automated on HTS liquid handling systems
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