What is Tris-EDTA Buffer used for?

Tris-EDTA Buffer is widely used in molecular biology and biochemistry, specifically in applications such as DNA and RNA extraction, storage, and electrophoresis. Its pH-stable environment helps to maintain nucleic acid integrity during experiments. Tris acts as a buffering agent while EDTA chelates divalent metal ions, which can otherwise degrade nucleic acids. For instance, researchers often employ this buffer when isolating genetic material to ensure optimal stability and performance.

What does 'Low EDTA' mean in this buffer?

'Low EDTA' indicates that the concentration of EDTA in the buffer is reduced compared to standard formulations. This adjustment helps minimize potential inhibition of downstream applications like PCR or sequencing, where excessive EDTA might interfere with enzyme activity. Accordingly, using a low EDTA version is favorable for labs focused on precision and reliability in their nucleic acid analyses.

Is this Tris-EDTA Buffer sterile?

Yes, this Tris-EDTA Buffer is sterile and 0.2 µm filtered to ensure that it is free from contaminants. Sterility is crucial in applications involving sensitive biological materials like DNA and RNA, as contamination could lead to experimental errors or degraded samples. Utilizing a sterile solution helps maintain the purity of your work, making it ideal for consistent and reproducible results in various molecular biology assays.

What is the pH level of this Tris-EDTA Buffer?

The pH level of this Tris-EDTA Buffer is set at 8.0, which is optimal for maintaining stability in nucleic acids. This pH is generally suitable for most biological applications, ensuring that enzymatic activities remain unaffected. For instance, researchers often choose this pH to facilitate efficient reactions in tools such as PCR, ensuring that the nucleotides and enzymes remain effective during the experimental process.

How is the Tris-EDTA Buffer used in DNA extraction?

In DNA extraction, Tris-EDTA Buffer serves as a crucial component that helps to stabilize the extracted nucleic acids. The buffer maintains the appropriate pH and protects DNA from degradation by chelating metal ions that could otherwise cause hydrolysis. For example, during phenol-chloroform extraction, this buffer is utilized as a wash step to ensure the quality and integrity of the isolated DNA, resulting in high-yield and pure samples for downstream applications.

What is the significance of filter 0.2 µm in this buffer?

The 0.2 µm filtering process is essential for removing bacteria and particulate matter from the Tris-EDTA Buffer, ensuring its suitability for sensitive applications. This level of filtration guarantees that the buffer is free from contaminants that could affect experimental outcomes. For laboratory settings where precision is critical, using a filtered buffer helps maintain sample integrity, particularly in experiments using cell cultures or sensitive reagents.

What applications can I use this buffer for?

This Tris-EDTA Buffer is versatile and can be used for various applications including DNA and RNA extraction, storage, gel electrophoresis, and enzymatic reactions. Its reliable properties make it a staple in molecular biology labs. For example, it can be employed in cloning experiments where maintaining sample integrity is paramount, or in gel electrophoresis to separate nucleic acid fragments based on size.

Can this buffer be used for RNA work?

Absolutely! The Tris-EDTA Buffer is suitable for RNA work, as it prevents the degradation of RNA molecules during various procedures. The low EDTA concentration helps avoid inhibiting reactions reliant on divalent cations. For researchers focusing on RNA-based assays, this buffer can be utilized effectively in RNA isolation protocols to ensure high purity and yield of RNA, suitable for applications like qPCR or transcriptomic studies.

What size is the packaging of this Tris-EDTA Buffer?

This product is conveniently packaged in a 500 mL bottle, making it suitable for use in both small lab settings and larger projects. The generous volume ensures that researchers have ample buffer for repetitive experiments or extended protocols without the need for frequent reordering. Additionally, the size is practical for maintaining sterility and ease of use in laboratory environments.

Where can I buy 1X TE, Low EDTA, pH 8.0 - Tris-EDTA Buffer?

You can purchase the 1X TE, Low EDTA, pH 8.0 - Tris-EDTA Buffer from Ubuy in UK. Ubuy offers a reliable platform for acquiring high-quality laboratory supplies, ensuring you have the materials needed for your research and experiments.

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UFC Bio

1X TE, Low EDTA, pH 8.0 - Tris-EDTA Buffer - Sterile, 0.2um Filtered - 500mL

1X TE Buffer, Low EDTA, pH 8.0. Size: 500mL. Sterile, 0.2um filtered to ensure upmost quality.

UFC Bio

Item #: 68232362

1X TE, Low EDTA, pH 8.0 - Tris-EDTA Buffer - Sterile, 0.2um Filtered - 500mL

84% of respondents would recommend this to a friend

Item #: 68232362

GBP 63

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Purity Assurance

The Tris-EDTA buffer is sterile and 0.2um filtered, ensuring high purity levels suitable for sensitive biological applications.

Optimal pH Stability

Maintains a stable pH of 8.0, offering optimal conditions for various molecular biology procedures and reagent compatibility.

Convenient Volume

Delivered in a generous 500mL size, providing ample supply for multiple experiments, reducing the need for frequent reorders.

Product Details

1X TE Buffer, Low EDTA, pH 8.0
500mL size
Used in various molecular biological applications
Ideal for re-suspending or diluting purified DNA/RNA
Sterile and 0.2um filtered for top quality
2-year expiry from the manufacturing date

Container Type	Bottle
Item Form	Liquid
Volume	500 ml
Brand Name	UFC Bio
Manufacturer	UFC Bio
UPC	791689523767
Included Components	1X TE Buffer, Low EDTA
Part Number	BTELE-01X
Brand	UFC Bio
Item Volume	500 ml
Package Information	Bottle
Item Weight	1.1 lbs (500 grams)

Who Should Buy?

Molecular Biologists
Ideal for DNA and RNA extraction, providing a stable environment for nucleic acid handling and storage.
Laboratory Research
Suitable for various enzymatic reactions and molecular analyses requiring a buffer with low metal ion contamination.
Clinical Testing
Sterile and filtered, perfect for use in clinical and diagnostic laboratories where contamination must be minimized.

Protein Studies
Not ideal for protein purification due to potential interference from EDTA with certain protein assays.

Product Description

1X TE, Low EDTA, pH 8.0 - Tris-EDTA Buffer - Sterile, 0.2um Filtered - 500mL

About This Item

Introducing our 1X TE Buffer, Low EDTA, pH 8.0 - Tris-EDTA Buffer. This sterile, 0.2um filtered solution is a must-have for any laboratory or research facility. With a size of 500mL, this product provides you with an ample supply for your experiments and studies. TE buffer, also known as Tris-EDTA Buffer, is commonly used in molecular biology procedures, especially in DNA and RNA research.

It provides a stable environment for nucleic acids, ensuring their integrity and preventing degradation. Our 1X TE Buffer has a low EDTA concentration, which is essential for certain applications where higher concentrations of EDTA could interfere with enzymatic reactions. The pH level of 8.0 ensures optimal performance and compatibility with a wide range of protocols and techniques. With this Tris-EDTA Buffer pH 8.0, you can confidently perform various molecular biology techniques such as DNA and RNA isolation, restriction enzyme digestion, and polymerase chain reaction (PCR). It is also suitable for DNA sequencing, genotyping, and cloning. Not only is our 1X TE Buffer highly effective, but it is also conveniently sterile and 0.2um filtered.

This ensures the elimination of any contaminants that could compromise your results. You can trust in the quality and purity of our product. Invest in the best with our 1X TE Buffer, Low EDTA, pH 8.0 - Tris-EDTA Buffer. Whether you are conducting research, performing diagnostic tests, or analyzing genetic material, this product is an essential addition to your laboratory supplies.

Don't settle for anything less than the highest quality when it comes to your scientific endeavors. Order your 500mL bottle today and experience the difference for yourself.

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Question: How to Shop 1X TE, Low EDTA, pH 8.0 - Tris-EDTA Buffer - Online From Ubuy?

Answer: It’s easy to shop 1X TE, Low EDTA, pH 8.0 - Tris-EDTA Buffer - online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 1X TE, Low EDTA, pH 8.0 - Tris-EDTA Buffer - Available to Shop Online in UK?

Answer: Yes, at Ubuy UK this product is available for you to shop at a reasonable price. The 1X TE, Low EDTA, pH 8.0 - Tris-EDTA Buffer - is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

UFC Bio Buffers Editorial Review

1X TE, Low EDTA, pH 8.0 - Tris-EDTA Buffer - Sterile, 0.2um Filtered - 500mL is a high-quality buffer solution from UFC Bio, designed for various laboratory applications. This liquid buffer comes in a 500 ml bottle and is specifically formulated to maintain a stable pH of 8.0 while minimizing EDTA concentrations, making it ideal for sensitive experiments. Users appreciate its sterile filtration process, ensuring contamination-free usage. The bottle packaging is convenient and lightweight, which is a plus for easy handling in the lab. With its reliable performance, this buffer is a great choice for researchers and technicians alike.

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Pros

Sterile filtration for contamination-free usage
Stable pH of 8.0 for accuracy
Lightweight bottle for easy handling
Low EDTA concentration for sensitive applications
Convenient 500 ml volume for various uses

Cons

Limited information on alternative pH options available

Product Price History

Important information

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GBP 63

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Features & Benefits

pH 8.0
500mL size
Sterile and 0.2um filtered for quality
Useful for molecular biological applications
Made with 18.2 Ohm water
2 years of shelf life from manufactured date

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1X TE, Low EDTA, pH 8.0 - Tris-EDTA Buffer - Sterile, 0.2um Filtered - 500mL

GBP 63

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GBP 63

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