What is TAE Buffer and its primary applications?

TAE Buffer (Tris-Acetate-EDTA) is a commonly used buffer in molecular biology, primarily for DNA electrophoresis. It helps maintain a stable pH during the separation process, ensuring the integrity of nucleic acids. Researchers utilize TAE Buffer for applications like gel electrophoresis and PCR, where precise pH control is vital to obtaining reproducible results and enhancing DNA band resolution.

How should TAE Buffer be prepared or diluted for use?

To prepare TAE Buffer, mix the appropriate amounts of Tris, acetic acid, and EDTA in distilled water. For electrophoresis, the 1X working solution is typically used, which can be made by diluting the 50X stock solution. Following the manufacturer's instructions for preparation ensures optimal results, which is crucial for successful DNA analysis and research applications.

Can I use TAE Buffer for RNA electrophoresis?

While TAE Buffer is predominantly used for DNA electrophoresis, it can also be used for RNA. The buffer helps maintain the RNA's stability during gel electrophoresis, although some researchers prefer using TBE Buffer for RNA due to its lower ionic strength. Depending on your experiment, TAE can be a suitable choice, especially if DNA and RNA analysis are both being conducted.

What are the benefits of using a 50X TAE Buffer concentrate?

A 50X TAE Buffer concentrate is beneficial for saving storage space and allowing flexibility in dilutions. Researchers can easily prepare various working concentrations based on their specific experimental needs. This concentrated form also provides long shelf life when stored properly, making it convenient for laboratories that frequently conduct DNA analysis.

Is TAE Buffer suitable for southern blotting techniques?

Yes, TAE Buffer can be utilized in southern blotting techniques. It aids in the separation and transfer of DNA fragments onto a membrane, which is essential for further processing and analysis such as hybridization. Researchers often prefer using TAE due to its ability to maintain the integrity of the DNA during the blotting process.

How does TAE Buffer compare to TBE Buffer?

TAE Buffer and TBE Buffer both serve as buffers during electrophoresis but have different ionic strengths and properties. TAE Buffer is generally used for DNA analysis, while TBE Buffer is preferred for high-performance applications. Choosing between them depends on specific experimental requirements, such as the type of nucleic acid and the resolution needs of your gel.

What is the role of EDTA in TAE Buffer?

EDTA (Ethylenediaminetetraacetic acid) in TAE Buffer chelates divalent cations like magnesium and calcium, which can inhibit enzymatic reactions. This is particularly important if you are working with nucleases that could degrade DNA. Maintaining a low concentration of these ions helps protect your DNA samples during experiments.

Can TAE Buffer be reused after electrophoresis?

While it is technically possible to reuse TAE Buffer, it is generally not recommended due to potential contamination from nucleic acids and proteins that may have migrated during electrophoresis. Reusing the buffer can lead to inconsistent results, so preparing fresh buffer for every experiment is advisable for maintaining experimental integrity.

What storage conditions are recommended for TAE Buffer?

TAE Buffer should be stored in a cool, dark place to prevent degradation. Ideally, it should be kept at room temperature and tightly sealed to avoid contamination. This helps in maintaining its effectiveness for prolonged periods, allowing researchers to rely on its consistency throughout their experiments.

Where can I buy 50x TAE Buffer (1 L) in UK?

You can buy 50x TAE Buffer (1 L) from Ubuy, which offers a wide range of laboratory supplies and reagents. The platform provides various options that cater to the needs of researchers and labs looking for quality products, making it a dependable source for your laboratory needs.

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Recommended for resolution of RNA and DNA fragments larger than 1500 bp.

Item #: 97964210

50x TAE Buffer (1 L)

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Item #: 97964210

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Recommended for resolution of RNA and DNA fragments larger than 1500 bp.

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What Stands Out

High Concentration

The 50x TAE buffer offers a concentrated formula ideal for various molecular biology applications, ensuring efficient electrophoresis and better resolution.

Cost-Effective

Available in a 1 L volume, it provides excellent value by reducing the need for frequent purchases while maintaining high-quality performance.

Versatile Use

Designed for versatile applications including DNA agarose gel electrophoresis, it simplifies laboratory workflows by serving multiple research purposes.

Product Details

Brand Name	Generic
Manufacturer	Bioland Scientific LLC
Included Components	buffer
Model Number	TAE01-1L
Part Number	TAE01-1L
Item Weight	2 lbs (910 grams)

Who Should Buy?

Biotechnology Labs
Ideal for laboratories conducting electrophoresis for DNA and RNA analysis, providing reliable performance in experiments.
Educational Institutions
Perfect for schools and universities teaching molecular biology, offering a durable and easy-to-use buffer solution.
Research Projects
Supports researchers in genetic studies, enhancing the accuracy and efficiency of nucleic acid separation techniques.

General Use
Not suitable for everyday tasks outside scientific research, as it is specific to molecular biology applications.

Product Description

50x TAE Buffer (1 L)

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Question: How to Shop 50x TAE Buffer (1 L) Online From Ubuy?

Answer: It’s easy to shop 50x TAE Buffer (1 L) online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 50x TAE Buffer (1 L) Available to Shop Online in UK?

Answer: Yes, at Ubuy UK this product is available for you to shop at a reasonable price. The 50x TAE Buffer (1 L) is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

High purity for reliable results
Convenient 1 L packaging
consistent performance in electrophoresis
Ready-to-use solution saves time
Excellent for DNA/RNA analysis

Cons

Occasional sediment formation

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GBP 62

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Features & Benefits

50X TAE Buffer is essential for nucleic acids electrophoresis.
Suitable for agarose and polyacrylamide gel applications.
Effective as both a running buffer and gel preparation buffer.
Filtered through a 0.22 µm membrane for optimal purity.
Ideal for genomic and large supercoiled DNA.
Perfect for resolving RNA and DNA fragments over 1500 bp.

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50x TAE Buffer (1 L)

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