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Item #:
36217259
Water, Molecular Biology Grade, RNase and Nuclease Free, 100 Milliliters
Item #:
36217259
GBP 39
Price Details
Price
GBP 39
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If Item price + shipping is below GBP 135, 20% VAT will be calculated on checkout, if it is equal or above GBP 135, no VAT will be charged. )
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Certified RNase and DNase free for use in molecular biology applications.
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What Stands Out
High Purity Grade
This water is certified Molecular Biology Grade, ensuring it is free from RNase and nuclease contamination, making it ideal for sensitive molecular biology applications and experiments.
Versatile Usage
Perfect for a variety of laboratory processes, including DNA/RNA extraction and purification, the RPI W82000-100.0 meets the rigorous demands of academic and industrial research settings.
Convenient Volume
With a 100mL size, this water provides an ample supply for experiments without excessive wastage, allowing researchers to efficiently manage their resources while maintaining high quality.
Product Details
| Item Weight | 0.2 lbs (90 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for molecular biologists conducting experiments that require pure water without RNase and nuclease contamination.
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Laboratory Technicians
Beneficial for technicians who need reliable, high-quality water for various laboratory procedures and assays.
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Genetic Research
Supports researchers in genetic studies, ensuring the integrity of nucleic acids during experiments and sample preparations.
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Everyday Use
Not suitable for general household use where molecular-grade water purity is unnecessary and impractical.
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Non-Scientific Purposes
Not recommended for personal or non-scientific projects that do not require laboratory-grade water purification.
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Large Scale Production
Not ideal for large-scale applications as the 100mL quantity limits its use for bulk experimental setups.
Product Description
Water, Molecular Biology Grade, RNase and Nuclease Free, 100 Milliliters
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is RPI W82000-100.0 Water used for in molecular biology?
Answer: RPI W82000-100.0 Water is primarily used in molecular biology applications where high purity is essential. This water is RNase and nuclease free, making it ideal for sensitive experiments such as PCR, cloning, and sequencing. The absence of RNases and nucleases helps prevent unwanted degradation of RNA and DNA, which is crucial for obtaining accurate and reproducible results. Researchers often prefer this grade of water for dilution of reagents, preparation of buffers, or any procedure where contaminating enzymes may interfere with the analysis. -
Question:
Is RPI W82000-100.0 Water sterile?
Answer: RPI W82000-100.0 Water is not classified as sterile but is designated as molecular biology grade, ensuring it is free from contaminants that could affect experimental integrity. For applications requiring sterility, researchers typically utilize autoclaving or filtration methods following their protocols. While this water offers significant purity by removing ribonucleases and deoxyribonucleases, handling it in a clean environment is paramount when preparing microbial cultures or handling biological samples. -
Question:
How should RPI W82000-100.0 Water be stored?
Answer: To maintain its quality, RPI W82000-100.0 Water should be stored in a cool, dark environment, ideally at temperatures between 2°C and 8°C. Exposure to light and heat can degrade water quality and increase the risk of contamination. Additionally, the container should remain sealed to prevent any airborne contamination. When required, aliquoting into smaller containers can help minimize the risk of repeated exposure to contaminants, ensuring the water remains suitable for high-fidelity applications. -
Question:
Can RPI W82000-100.0 Water be used in preparing buffers?
Answer: Yes, RPI W82000-100.0 Water is well-suited for preparing various types of buffers used in molecular biology experiments. Its high purity level ensures that it does not introduce unnecessary ions or contaminants that could affect buffer performance. Common uses include making Tris, PBS, and other aqueous solutions critical for cell culture experiments and enzymatic reactions. The consistent quality of this water allows researchers to accurately replicate buffer conditions across different experiments. -
Question:
What are the applications of RNase-free water in laboratories?
Answer: RNase-free water like RPI W82000-100.0 is critical in laboratories, especially in molecular biology and bioanalysis. It's routinely used for RNA and PCR work where contamination can lead to erroneous results. Additionally, it's ideal for creating dilutions, washing reagents, and preparing solutions essential in various analyses such as qPCR and Northern blotting. The assurance of RNase and nuclease freedom supports successful outcome metrics that are vital in academic research and clinical diagnostics. -
Question:
What does RNase and nuclease-free mean?
Answer: The terms RNase and nuclease-free indicate that the water has been processed to eliminate enzymes that can degrade RNA and DNA. This is particularly important for experiments involving nucleic acids since even trace amounts of these enzymes can compromise the integrity of samples. RPI W82000-100.0 Water is specifically designed to provide this level of purity, which is essential for applications like gene expression studies, where RNA stability is crucial for obtaining reliable data. -
Question:
Is RPI W82000-100.0 Water suitable for cell culture?
Answer: While RPI W82000-100.0 Water is primarily intended for molecular biology applications, it can also support cell culture under certain conditions. Its RNase and nuclease-free quality makes it safe for preparing solutions like media and buffers that require optimal conditions for cell growth. However, researchers should consider using additional supplements or additives as necessary for specific cell lines, as RPI W82000-100.0 does not include growth factors or nutrients. -
Question:
How can I verify the purity of RPI W82000-100.0 Water?
Answer: The purity of RPI W82000-100.0 Water can often be assessed through certificate of analysis provided with each batch, detailing test results for RNase, nuclease, and other contaminants. Many reputable suppliers conduct comprehensive testing to ensure adherence to molecular biology-grade specifications. For additional verification, techniques like gel electrophoresis can show the integrity of RNA or DNA when prepared using this water, serving as practical evidence of its purity in your experimental processes. -
Question:
Can RPI W82000-100.0 Water be used for making dilutions?
Answer: Absolutely, RPI W82000-100.0 Water is ideally suited for making dilutions in molecular biology protocols. Whether diluting enzymes, primers, or other reagents for experiments, its high purity minimizes the risk of reactions being influenced by contaminants. Researchers often utilize this water for preparing necessary concentrations while ensuring that results are consistent and reliable, particularly in quantitative assays like qPCR or enzyme assays where precise measurements are critical. -
Question:
Where can I buy RPI W82000-100.0 Water in UK?
Answer: You can purchase RPI W82000-100.0 Water, Molecular Biology Grade, RNase and Nuclease Free, 100mL from Ubuy. Ubuy provides a convenient online shopping experience, allowing you to find this high-purity water along with a wide range of laboratory supplies. Their platform is designed to cater to various needs in the scientific community, ensuring you have access to top-quality products required for your research and experiments.
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Pros
- High purity for sensitive experiments
- RNase and nuclease free
- Ideal for molecular biology applications
- consistent performance across batches
- Convenient 100mL packaging
Cons
- Slightly higher price than competitors
Product Price History
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Features & Benefits
- Ideal for making reagents, rinsing glass and plastic ware, enzymatic reactions and more.
- Toxic agents such as DEPC are not used in purification procedures.
- Each bottle has a 100 ml capacity.
- Guaranteed RNase free and DNase free.
- Suitable for a variety of molecular biology applications.
- Convenient for use in enzymatic reactions.